The development of in vitro techniques for production of haploids was a major feat in the fields of biotechnology and plant breeding in the past few decades. It is documented that Blakelsee et al. …

By applying growth regulator 2,4-dichlorophenoxyacetic acid in planta, embryo growth is maintained to the stage suitable for embryo isolation and further in vitro culture. The maize chromosome …

Herein, a protocol is described for the production of DHLs, valuable as parents for commercial long-fruit type cucumber hybrids. The protocol is based on in situ induced parthenogenesis and in...

Haploid plants obtained from anther cultures were colchicine treatment according to protocol developed for Capsicum annuum L. The apical parts of 106 androgenic haploids were placed for six days on the …

•Why is doubled haploid technology impactful for agriculture? •Doubled haploid technology comprises both the production of haploid plants and the chromosome doubling process (see Figure 1).

The production of pure lines using doubled haploids has several advantages over conventional methods. Using DH production systems, homozygosity is achieved in one generation, eliminating the need for …

Overview of in vitro and in vivo haploidization methods in plants. Intersection depicts in vivo methods that necessitate in vitro step(s) to prevent haploid embryo abortion.